Cataloguenumber
ANT-414
Synonyms
5"-nucleotidase,Ecto-5"-nucleotidase,5"-NT,CD73,NT5E,NT5,NTE,NT,eN,eNT,E5NT.
Type
MouseAntiHumanMonoclonal.
Introduction
Ecto-5-prime-nucleotidase(5-prime-ribonucleotidephosphohydrolase)catalyzestheconversionofpurine5-primemononucleotidestonucleosides,thepreferredsubstratebeingAMPataneutralpH.CD73,whichconsistsofadimerof2identical70kDasubunits,isboundbyaglycosylphosphatidylinositollinkagetotheexternalfaceoftheplasmamembrane.CD73isusedasa
Markeroflymphocytedifferentiation.Consequently,adeficiencyofNT5occursinavarietyofimmunodeficiencydiseases.Additionalformsof5-primenucleotidaseexistinthecytoplasmandlysosomesandcanbedistinguishedfromecto-NT5bytheirsubstrateaffinities,requirementfordivalentmagnesiumion,activationbyATP,andinhibitionbyinorganicphosphate.
Clone
P2B6AT.
Immunogen
Anti-humanCD73mAb,isderivedfromhybridizationofmouseFOmyelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanCD73aminoacids27-252purifiedfromE.coli.
IgSubclass
MouseIgG2bheavychainandκlightchain.
PurificationMethod
CD73antibodywaspurifiedfrommouseasciticfluidsbyprotein-Gaffinitychromatography.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
1mg/mlcontainingPBS,pH-7.4,&0.1%SodiumAzide.
StorageProcedures
Forperiodsupto1monthstoreat4°C,forlongerperiodsoftime,storeat-20°C.Preventfreezethawcycles.
StABIlity/ShelfLife
12monthsat-20°C.1monthat4°C.
Applications
CD73antibodyhasbeentestedby
ELISAandWesternblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.RecommendeddilutionrangeforWesternblotanalysisis1:1,000~2,000.Recommendedstartingdilutionis1:1,000.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.