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℡ 4000-520-616
℡ 4000-520-616
Prospec/TGFB3 Human/Transforming Growth Factor-Beta 3 Human Recombinant/10µg/CYT-368-10µg-10µg
产品编号:CYT-368-10µg-10µg
市  场 价:¥2600.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$130.00
品      牌: Prospec
公      司:Prospec-Tany
公司分类:
Prospec/TGFB3 Human/Transforming Growth Factor-Beta 3 Human Recombinant/10µg/CYT-368-10µg-10µg
商品介绍

Catalogue number

CYT-368

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Introduction

Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

Description

TGF-β 3 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 113 amino acid chains and having a total molecular mass of 25.8kDa. The TGF-β 3 is purified by standard chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The protein solution contains 20% Ethanol and 10mM Acetic acid (AcOH).

Stability

TGF-beta 3 although stable at room temperature for 1 week, should be stored at 4°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Purity

Greater than 95.0% as determined by SDS-PAGE.

Amino acid sequence

MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.

Biological Activity

The activity is determined by the ability to induce chondrogenic differentiation.

Protein content

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.

References

1.Title: Altering the Architecture of Tissue Engineered Hypertrophic Cartilaginous Grafts Facilitates Vascularisation and Accelerates MineralisationPublication: DOI: 10.1371/journal.pone.0090716, March 04, 2014Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0090716
2.Title: Oxygen tension differentially regulatesthe functional properties of cartilaginous tissues engineered frominfrapatellar fat pad derived MSCs and articular chondrocytesPublication: Osteoarthritis and Cartilage 18.10 (2010):1345-1354.Link:http://www.sciencedirect.com/science/article/pii/S1063458410002293

Safety Data Sheet

SDS

Usage

Prospec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。

 

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