Cataloguenumber
ENZ-056
Synonyms
Onitase1Soluble,IRP1,IREB1,IREBP,Citratehydro-lyase,Ironregulatoryprotein1,Ferritinrepressorprotein,Iron-responsiveelement-bindingprotein1,ACONS,AconitateHydratase,EC4.2.1.3,Aconitase.
Introduction
ACO1hasapartinanironsensor.ACO1catalyzesthestereo-specificisomerizationofcitratetoisocitrateviacis-aconitateinthetricarboxylicacidcycle,anon-redox-activeprocess.
Description
ACO1producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining912aminoacids(1-889a.a.)andhavingamolecularmassof100.8kDa.ACO1isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
TheACO1proteinsolution(0.5mg/1ml)isformulatedin20mMTris-HClbuffer(pH8.0)2mMDTT,100mMNaCland10%glycerol.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELD
LKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleaseavoidfreezethawcycles.
SafetyDataSheet
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.