Cataloguenumber
PRO-1178
Synonyms
Nucleolarprotein3(apoptosisrepressorwithCARDdomain),Muscle-enrichedcytoplasmicprotein,Nucleolarproteinof30kDa,ApoptosisrepressorwithCARD,nucleolarprotein3,NOP30,CARD2,ARC,NOP,MYP,MYC.
Introduction
Nucleolarprotein3(NOL3)isprimarilyexpressedinthemuscleandisappearsinthenucleoplasmandconcentratedinnucleoli.NOL3isananti-apoptoticproteinwhichdown-regulatestheenzymaticactivitiesofcaspase2,caspase8andtumorproteinp53.NOL3ishighlyexpressedintheheartandskeletalmuscle.NOL3isidentifiedatlowlevelsintheplacenta,liver,kidneyandpancreas.TheNOL3mRNAandproteinlevelsarestimulatedbyneuronalactivity,whichisvitalinordertopromoteneuroplasticity,thusindicatingapotentialroleforNOL3inactivity-dependentchangesindendritefunction.NOL3localizestothe
CytoskeletonofneuronalcellsandseemstocolocalizewithF-Actin,thoughitmayconnectwithanactin-associatedproteininsteadofdirectlywithF-Actin.
Description
NOL3HumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining231aminoacids(1-208)andhavingamolecularmassof25.0kDa.
NOL3isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheNOL3solution(1mg/1ml)contains20mMTris-HClbuffer(pH8.0),100mMNaCl,1mMDTT,1mMEDTAand10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMGNAQERPSETIDRERKRLVETLQADSGLLLDALLARGVLTGPEYEALDALPDAERRVRRLLLLVQGKGEAACQELLRCAQRTAGAPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPGSGTTCPGLPRASDPDEAGGPEGSEAVQSGTPEEPEPELEAEASKEAEPEPEPEPELEPEAEAEPEPELEPEPDPEPEPDFEERDESEDS.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.