Cataloguenumber
PRO-875
Synonyms
RAB3BmemberRASoncogenefamily,brainantigenRAB3B,ras-relatedproteinRab-3B.
Introduction
RAB3BwhichbelongstotheRASoncogenefamilyisanextremelyhomologousmonomericGTPasethattakespartinendocytosisandbiosyntheticproteintransport.ThetransportofrecentlysynthesizedproteinsfromtheendoplasmicreticulumtoseveralstacksoftheGolgicomplexandtosecretoryvesiclesincludesineveryphasethemovementofcarriervesicles.
Description
RAB3BHumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining239aminoacids(1-219)andhavingamolecularmassof26.9kDa.
TheRAB3Bisfusedtoa20aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
RAB3Bissuppliedasasterilefilteredclearsolution.
Formulation
RAB3Bprotein0.5mg/mlissuppliedin20mMTris-HCl,pH-8,0.1MNaCl,2mMDTTand20%Glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMASVTDGKTGVKDASDQNFDYMFKLLIIGNSSVGKTSFLFRYADDTFTPAFVSTVGIDFKVKTVYRHEKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWATQIKTYSWDNAQVILVGNKCDMEEERVVPTEKGQLLAEQLGFDFFEASAKENISVRQAFERLVDAICDK
MSDSLDTDPSMLGSSKNTRLSDTPPLLQQNCSC.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.