Cataloguenumber
PRO-935
Synonyms
Ras-relatedproteinRab-35,GTP-bindingproteinRAY,Ras-relatedproteinRab-1C,RAB35,RAB1C,RAY.
Introduction
Ras-relatedproteinRab-35(RAB35)isrestrictedtotheplasmamembraneandendocyticcompartmentsandcontrolsafastendocyticrecyclingpathway.InhibitionofRab35functionleadstotheaccumulationofendocytic
Markersonnumerouscytoplasmicvacuolesincellsthatfailedcytokinesis,whichisconsistentwithacentralrequirementforRab35-regulatedrecyclingduringcelldivision.
Description
RAB35HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining221aminoacids(1-201a.a.)andhavingamolecularmassof25.2kDa.
RAB35isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
RAB35proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),40%glycerol,0.15MNaCland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC.
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.