Cataloguenumber
PRO-950
Synonyms
RAB32memberRASoncogenefamily,ras-relatedproteinRab-32.
Introduction
RAB32isamemberofthesmallGTPasesuperfamily.RAB32controlsERcalciummanagementanddisturbsthespecificenhancementofcalnexinontheMAM(mitochondria-associatedmembrane),howeveravoidsaffectingtheERdistributionofprotein-disulfideisomeraseandmitofusin-2.Fur
Thermore,RAB32regulatesthetargetingofPKA(cAMP-dependentproteinkinase)tomitochondrialandERmembranesandbyinactivationoroverexpressionenhancesthephosphorylationofDrp1andBad.UsingacombinationofitsfunctionsasaregulatorofMAMpropertiesandaPKA-anchoringprotein,theactivityandexpressionlevelofRAB32definethespeedofapoptosisonset.
Description
RAB32HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining249aminoacids(1-225)andhavingamolecularmassof27.6kDa.
RAB32isfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheRAB32solution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),5mMDTT,0.2MNaCl,0.2MEDTAand50%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.