Cataloguenumber
PRO-1013
Synonyms
Ras-relatedproteinRab-2B,RAB2B.
Introduction
Rab2BisamemberofthesmallGTPasesuperfamily,whosemembersarenontransformingmonomericGTP-bindingproteinsoftheRassuperfamilyandcontain4highlyconservedregionsinvolvedinGTPbindingandhydrolysis.Rabproteinsareprenylated,membrane-boundproteinsengagedinvesicularfusionandtrafficking.
Description
RAB2BHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining240aminoacids(1-216a.a.)andhavingamolecularmassof26.8kDa.
RAB2Bisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
RAB2Bproteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),10%glycerol,1mMDTTand0.15MNaCl.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMTYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMVNIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFNHLTSWLEDARQHSSSNMVIMLIGNKSDLESRRDVKREEGEAFAREHGLIFMETSAKTACNVEEAFINTAKEIYRKIQQGLFDVHNEANGIKIGPQQSISTSVGPSASQRNSRDIGSNSGCC.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.