Cataloguenumber
PRO-045
Synonyms
Ras-relatedproteinRab-1B,RAB1B.
Introduction
RAB1BbelongstotheRABproteinfamily,theseproteinsarelowmolecularmassmonomericGTPaseslocalizedonthecytoplasmicsurfacesofdistinctmembraneboundorganelles.RAB1Bproteinfunctionsintheearlysecretorypathwayandisessentialforvesicletransportbetweentheendoplasmicreticulum(ER)andGolgi.
Description
RAB1BHumanRecombinantfusedwitha20aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining221aminoacids(1-201a.a.)andhavingamolecularmassof24.3kDa.TheRAB1Bispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheRAB1Bsolution(1mg/ml)contains20mMTris-HClbuffer(pH8.0)and10%Glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.