Cataloguenumber
ENZ-558
Synonyms
HH13,HH7-2,HU-PP-1,OV-1,PRL-2,PRL2,ptp-IV1a,ptp-IV1b,PTP4A,PTPCAAX2.
Introduction
PTP4A2ispartofasmallclassoftheproteintyrosinephosphatase(PTP)family.PTP4A2islocalizedtotheearlyendosomethattakepartinavarietyofcellularactivities.PTP4A2proteininteractswiththebeta-subunitofRabgeranylgeranyltransferaseII(betaGGTII),thereforehasaroleasaregulatorofGGTIIactivity.OverexpressionofPTP4A2inmammaliancellsconferredatransformedphenotype,whichsuggesteditsroleintum
Origenesis.
Description
RecombinantHumanPTP4A2producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining203aminoacids(1-167a.a.)andhavingamolecularmassof23.2kDa.PTP4A2isfusedtoa36aminoacidHis-TagatN-Terminusandpurifiedbyconventionalchromatographytechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
ThePTP4A2(0.5mg/ml)proteinsolutioncontains20mMTris-HCl,pH-8,1mMEDTAand10%Glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMNRPAPVEISYENMRFLITHNPTNATLNKFTEELKKYGVTTLVRVCDATYDKAPVEKEGIHVLDWPFDDGAPPPNQIVDDWLNL
LKTKFREEPGCCVAVHCVAGLGRAPVLVALALIECGMKYEDAVQFIRQKRRGAFNSKQLLYLEKYRPKMRLRFRDTNGHCCVQ.
SafetyDataSheet
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。