GCE,G
CST,GCVT,NKH,Aminomethyltransferase,mitochondrial,GlycinecleavagesystemTprotein,GCVT.
Aminomethyltransferase,mitochondrialisoform1(AMT)isacomponentoftheglycinecleavagesystemtermedT-protein.AMTrevers
IBLycatalyzesthedegradationoftheaminomethylmoietyofglycineattachedtothelipoatecofactorofH-protein,le
ADIngtotheproductionofammonia,5,10-methylenetetrahydrofolate,anddihydrolipoate-bearingH-proteininthepresenceoftetrahydrofolate.
AMTHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining398aminoacids(29-403a.a)andhavingamolecularmassof43.3kDa.AMTisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
EscherichiaColi.
SterileFilteredcolorlesssolution.
AMTproteinsolution(1mg/ml)inPhosphateBufferedSaline,30%glyceroland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Greaterthan90.0%asdeterminedbySDS-PAGE.
MGSSHHHHHHSSGLVPRGSHMGSAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRV
TRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.