Cataloguenumber
PRO-874
Synonyms
RAB17memberRASoncogenefamily,ras-relatedproteinRab-17,FLJ12538.
Introduction
RAB17isamemberofthesmallGTPasesuperfamilywhicharefoundinseveralintracellularcompartmentsandhaveasignificantregulatorypartinmembranetrafficking.RAB17regulatestrafficthroughtheapicalrecyclingendosome,whichindicatesthatRAB17hasaroleinpolarizedarrangementinepithelialcells.
Description
RAB17HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining232aminoacids(1-212)andhavingamolecularmassof25.6kDa.
TheRAB17isfusedtoa20aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
RAB17issuppliedasasterilefilteredclearsolution.
Formulation
RAB17protein0.5mg/mlissuppliedin20mMTris-HCL,pH-8,0.1MNaCl,1mMDTTand30%Glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAQAHRTPQPRAAPSQPRVFKLVLLGSGSVGKSSLALRYVKNDFKSILPTVGCAFFTKVVDVGATSLKLEIWDTAGQEKYHSVCHLYFRGANAALLVYDITRKDSFLKAQQWLKDLEEELHPGEVLVMLVGNKTDLSQEREVTFQEGKEFADSQKLLFME
TSAKLNHQVSEVFNTVAQELLQRSDEEGQALRGDAAVALNKGPARQAKCCAH
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.