Cataloguenumber
PRO-1796
Synonyms
MAGED1,MelanomaAntigenFamilyD1,NeurotrophinReceptor-Interacting,MAGEHomolog,NRAGE,MAGETumorAntigenCCF,MAGE-D1Antigen,DLXIN-1,Melanoma-AssociatedAntigenD1.
Introduction
Melanoma-associatedantigenD1(MAGED1)belongstothemelanomaantigengene(MAGE)familyandexpressedinvirtuallyallnormaladulttissues.MAGED1isinvolvedinthep75neurotrophinreceptormediatedprogrammedcelldeathpathway.MAGED1isinvolvedinPrader-Willisyndromeincludinghyperphagia,repetitiveandcompulsivebehaviors,andcognitiveimpairment.MAGED1isinvolvedintheapoptoticresponsefollowingNGF(nervegrowthfactor)bindinginneuronalcells.MAGED1hinderscellcycleprogression,andfacilitatesNGFR-mediatedapoptosis.MAGED1functionsasaregulatorofthefunctionofDLXfamilymembers.MAGED1hasaroleinthecirc
ADIanrythmregulation.
Description
MAGED1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining280aminoacids(504-760a.a)andhavingamolecularmassof31.7kDa.
MAGED1isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
MAGED1proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glyceroland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGP.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.