Cataloguenumber
PRO-2206
Synonyms
MelanomaAntigenFamilyA8,MAGE-8Antigen,MAGE8,Cancer/TestisAntigen1.8,CT1.8,Cancer/TestisAntigenFamily1,Member8,Cancer/TestisAntigenFamily1,Melanoma-AssociatedAntigen8,MelanomaAntigenFamilyA,8,Member8,MAGEA8.
Introduction
MelanomaAntigenFamilyA8,alsoknownasMAGEA8belongstotheMAGEgenefamily,whichincludestwelveknowngenes,ofwhichsixareexpressedintumors.Fur
Thermore,theMAGEgeneswereatfirstisolatedfromdifferentkindsoftumors,andaccordingtotheirvirtuallyexclusivetumor-specificexpressioninadulttissues,theyhavebeenusedastargetsforcancerimmunotherapy.
Description
MAGEA8HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining341aminoacids(1-318a.a)andhavingamolecularmassof37.6kDa.
MAGEA8isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
MAGEA8proteinsolution(0.25mg/ml)containingPhosphateBufferedSaline,10%glyceroland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMLLGQKSQRYKAEEGLQAQGEAPGLMDVQIPTAEEQKAASSSSTLIMGTLEEVTDSGSPSPPQSPEGASSSLTVTDSTLWSQSDEGSSSNEEEGPSTSPDPAHLESLFREALDEKVAELVRFLLRKYQIKEPVTKAEMLESVIKNYKNHFPDIFSKASECMQVIFGIDVKEVDPAGHSYILVTCLGLSYDGLLGDDQSTPKTGLLIIVLGMILMEGSRAPEEAIWEALSVMGLYDGREHSVYWKLRKLLTQEWVQENYLEYRQAPGSDPVRYEFLWGPRALAETSYVKVLEHVVRVNARVRISYPSLHEEALGEEKGV.
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.