Cataloguenumber
PRO-843
Synonyms
CT1.4,MAGE4,MAGE-4antigen,MAGE-X2antigen,MAGE-41antigen,MAGE4B,MGC21336,Cancer/testisantigen1.4,MAGEA4.
Introduction
MAGEA4ispartoftheMAGEAfamily,whichdirectstheexpressionoftumorantigensthatareidentifiedonahumanmelanomabyautologouscytolyticTlymphocytes(CTL).MAGEA4participatesinembryonaldevelopmentandtumortransformationoraspectsoftumorprogression.MAGEA4isexpressedinmanytumorsofseveraltypes,suchasmelanoma,headandnecksquamouscellcarcinoma,lungcarcinomaandbreastcarcinoma,butnotinnormaltissuesexceptfortestesandplacenta.
Description
MAGEA4RecombinantHumanproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining337aminoacids(1-317a.a.)andhavingamolecularmassof37kDa.TheMAGEA4isfusedtoa20aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
MAGEA4Humansolutioncontaining20mMTris-HClpH-8&20%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV.
Usage
Prospec"sproductsarefurnishedfor LABORATORYRESEARCH USE ONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.