Cataloguenumber
PRO-1888
Synonyms
DRB1,HLADRB1,HLA-DR1B,HLA-DRB1,MHCclassIIantigenDRB116,DR-16,DR16,HumanLeucocyteAntigenDRB1,MHCClassIIHLA-DR-BetaCellSurfaceGlycoprotein,MHCClassIIHLA-DRw10-Beta.
Introduction
MajorHistocompatibilityComplexClassIIDRBeta1alsoknownasHLA-DRB1iaamemberoftheHLAclassIIbetachainparalogs.MoleculeclassIIisaheterodimerconsistingofanalpha(DRA)andabetachain(DRB),bothanchoredinthemembrane.HLA-DRB1takesanessentialpartintheimmunesystembypresentingpeptidesderivedfromextracellularproteins.ClassIImoleculesareexpressedinantigenpresentingcells(APC:Blymphocytes,dendriticcells,macrophages).Fur
Thermore,thebetachainisapproximately26-28kDa.Itisencodedby6exons.Whileexononeencodestheleaderpeptide;exons2and3encodethetwoextracellulardomains;exon4encodesthetransmembranedomain;andexon5encodesthecytoplasmictail.
Description
HLA-DRB1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining219aminoacids(30-227a.a)andhavingamolecularmassof25.2kDa.
HLA-DRB1isfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
HLA-DRB1proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),0.4MUreaand10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleaseavoidfreezethawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSK
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.