Prospec/LLO PEST free/PRO-373/50µg,Prospec,,Prospec,prospecbio,ProSpec-Tany,进口Prospec品牌清关代理 ProSpec h-ANP试剂盒,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Prospec/LLO PEST free/PRO-373/50µg

产品编号：PRO-373

市场价：¥8000.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$400.00

品牌： Prospec

公司：Prospec-Tany

公司分类：

商品介绍

Cataloguenumber

PRO-373

Synonyms

Listeriolysin-O,LLO,hlyA.

Introduction

ListeriolysinO(akaLLO)isahemolysinproducedbyListeriamonocytogenesbacteria,thepathogenresponsIBLeforcausinglisteriosis.Thetoxinmayberegardedasavirulencefactor,sinceitiscrucialforthevirulenceofL.monocytogenes.LLOisasinglepolypeptideproteinencodedbythehlyAgeneandcomposedof529residues.LLOisathiol-activatedcholesterol-dependentporeformingtoxinprotein;therefore,itisactivatedbyreducingagentsandinhibitedbyoxidizingagents.Still,LLOdiffersfromotherthiol-activatedtoxins,asitscytolyticactivityismaximizedatapHof5.5.Insidetheacidicphagosomes(averagepH~5.9)ofcellsthathavephagocytosedL.monocytogenes,LLOisselectivelyactivatedbymaximizingactivityatapHof5.5.FollowingthephagosomelysisbyLLO,thebacteriumbreaksoutintothecytosol,whereitisabletogrowintracellularly,andthetoxinhasreducedactivityinthemorebasiccytosol.Thus,LLOpermitsL.monocytogenestobreakoutfromthephagosomesintothecytosolwithoutharmingtheplasmamembraneoftheinfectedcell,whichallowsthebacteriatoliveintracellularly,wheretheyareshelteredfromextracellularimmunesystemfactorssuchasthecomplementsystemandantibodies.LLOalsobringsaboutdephosphorylationofhistoneH3anddeacetylationofhistoneH4intheearlyphasesofinfection,beforeentryofL.monocytogenesintothehostcell.Thepore-formingactivityisnotimplicatedincausingthehistonemodifications.Themodificationsofthehistonesaffectthedownregulationofgenesencodingproteinsinvolvedintheinflammatoryresponse.Therefore,LLOmaybesignificantinsubvertingthehostimmuneresponsetoL.monocytogenes.AtitsNH2-terminusitpossessesa25residueslongtypicalsignalsequenceexcitedduringthesecretionprocess.Moreover,initsNH2-terminusthereisalsoa19aminoacidsPEST-likesequencethatmaytargetthistoxinfordegradation.ThePEST-likesequencefoundinLLOandisconsideredcrucialforvirulence,giventhatmutantslackingthesequencelysedthehostcell.Nevertheless,contrarytoPEST"ssupposedroleinproteindegradation,evidenceimpliesthatthePEST-likesequencemaycontrolLLOproductioninthecytosolratherthanincreasedegradationofLLO.

Description

RecombinantListeriolysinOlackingboththesignalsecretionsequenceandthePEST-likesequence.LLO-PESTminusiscomposedof471aminoacids,startingfromaminoacid60toaminoacid529,withtheadditionofamethionineinitsNH2-terminus.

Source

EscherichiaColi.

PhysicalAppearance

SterileFilteredclearsolution.

Formulation

Theproteincontains50mMNaH2PO4,1mMEDTA,2.7mMKCl,1mMDTT,5%glyceroland0.5MNaCl.

StABIlity

Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Avoidmultiplefreeze-thawcycles.

Purity

Greaterthan90.0%asdeterminedbySDS-PAGE.

BIOLOGicalActivity

7x10⁴ HU/mg.2mMDTTcouldbeusetoreactivatethetoxin.

Applications

a.Cytosolicdeliveryofmolecules,peptides,oligonucleodidesandplasmidDNA(4).
b.Productionofspecificmonoclonalantibodies(5).
c.Detectionofanti-listeriolysinOantibodies(6).

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。