Cataloguenumber
PRO-1252
Synonyms
Proteinlin-7homologA,Lin-7A,hLin-7,Mammalianlin-sevenprotein1,MALS-1,Taxinteractionprotein33,TIP-33,Vertebratelin-7homolog1,Veli-1,LIN7A,MALS1,VELI1,LIN7.
Introduction
Lin7Aisamemberofthelin-7family.Lin7AiscomprisedofoneL27domainandonePDZ(DHR)domain.Lin7Aplaysapartinlaunchingandmaintainingtheasymmetricdistributionofchannelsandreceptorsattheplasmamembraneofpolarizedcells.Lin7Aisexpressedinthebrain,testis,kidney,placentaandliver.
Description
LIN7AHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining256aminoacids(1-233a.a.)andhavingamolecularmassof28.4kDa.
LIN7Aisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
LIN7Aproteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),0.1MNaCl,10%glyceroland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMLKPSVTSAPTADMATLTVVQPLTLDRDVARAIELLEKLQESGEVPVHKLQSLKKVLQSEFCTAIREVYQYMHETITVNGCPEFRARATAKATVAAFAASEGHSHPRVVELPKTDEGLGFNVMGGKEQNSPIYISRIIPGGVAERHGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAKDSVKLVVRYTPKVLEEMEARFEKLRTARRRQQQQLLIQQQQQQQQQQTQQNHMS.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.