Leukocyte-associatedimmunoglobulin-likereceptor1,LAIR-1,mLAIR1,CD305,LAIR1.
LAIR1isaglycoproteinexpressedonthesurfaceofthemajorityofhumanperipheralbloodmononuclearleukocytesincludingTcells,Bcells,NKcells,macrophagesanddendriticcells.LAIR-1functionsasaninhibitoryreceptorinNKcells,TcellsandBcells.Inhibitoryreceptorscontroltheimmuneresponsetopreventlysisofcellsrecognizedasself.Lair1consistsofaleadersequence,extracellulardomain,transmembranedomain,cytoplasmicregion.
LAIR1isamemberofboththeimmunoglobulinsuperfamilyandtheleukocyte-associatedinhibitoryreceptorfamily.LAIR1mapstoaregionof19q13.4labeledtheleukocytereceptorcluster,whichcontainsatleast29genesencodingleukocyte-expressedreceptorsoftheimmunoglobulinsuperfamily.
LAIR1MouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining132aminoacids(22-144aa)andhavingamolecularmassof15kDa(Migratesat18-28kDaonSDS-PAGEunderreducingconditions).
LAIR1isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Sf9,Baculoviruscells.
Sterilefilteredcolorlesssolution.
LAIR1proteinsolution(0.5mg/ml)containsPhosphatebufferedsaline(pH7.4)and10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Greaterthan90.0%asdeterminedbySDS-PAGE.
ADPQEGSLPDITIFPNSSLMISQGTFVTVVCSYSDKHDLYNMVRLEKDGSTFMEKSTEPYKTEDEFEIGPVNETITGHYSCIYSKGITWSERSKTLELKVIKENVIQTPAPGPTSDTSWLKTYSIYHHHHHH
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.