Cataloguenumber
PRO-2037
Synonyms
LeukocyteCell-DerivedChemotaxin2,LeukocyteCell-DerivedChemotaxin-2,Chondromodulin-II,Chm-II,LECT-2,HLECT2,Chm2,LECT2.
Introduction
LeukocyteCell-DerivedChemotaxin2(LECT2)functionsasachemotacticfactortoneutrophils.LECT2stimulatestheproliferationofchondrocytesandosteoblasts.LECT2isstronglyexpressedintheliverandweaklyinthetestis.LECT2isasecreted,16kDaproteinwhichservesasachemotacticfactortoneutrophilsandstimulatesthegrowthofchondrocytesandosteoblasts.LECT2proteinhasahighsequencesimilaritytothechondromodulinrepeatregionsofthechickenmyb-inducedmyeloid1protein.ApolymorphismintheLECT2geneislinkedwithrheumatoidarthritis.
Description
LECT2HumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(Gly19-Leu151)containing143aminoacidsincludinga10aaHistagatN-terminus.Thetotalcalculatedmolecularmassis16kDa.
Source
EscherichiaColi.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
LECT2wasfiltered(0.4µm)andlyophilizedin20mMTrisbuffer,50mMNaCl&pH7.5.
Solubility
Itisrecommendedtoadd200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.LECT2isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Aminoacidsequence
MKHHHHHHASGPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVDILCSAGSTVYAPFTGMIVGQEKPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVHIENCDSSDPTAYL.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.