Cataloguenumber
PRO-1590
Synonyms
Lactotransferrin,Lactoferrin,Growth-inhibitingprotein12,Talalactoferrin,LTF,GIG12,LF,HLF2,NeutrophilLactoferrin.
Introduction
Lactoferrinisaglycoproteinthatbelongstothetransferrinfamilyofironbindingproteins.Itisfoundinhumanbreastmilkaswellasmostepithelialsurfacesecretionsincludingtears,nasogastric,saliva,andbronchial.Lactoferrinbinds2moleculesofironwithveryhighaffinity.Lactoferrininhibitsbacterialgrowthbywithholdingiron,itsN-terminalregionisanantimicrobialpeptide.Lactotransferrinactssynergisticallywithlysozymetopotentiatetheactivityofbothproteins.Themultifunctionalproteinlactoferrinhasmanyphysiologicalposs
IBLeroles.Itisoftenreferredtoasaninnatedefenseproteinandfrequentlyservesasthefirstlineofdefenseinprotectionagainstpathogens.Ithasbeenshowntohavethe
ABIlitytobindiron,itisanaturalanti-bacterial,anti-fungalandanti-viral,itisanantioxidantanditalsohasimmunomodulatoryproperties.Ithasmanybeneficialproperties,whichmakeitagoodcandidateforanumberofproductapplications.Considerableresearchiscurrentlygoingontoexplainthevarioussuggested
BIOLOGicalfunctionsoflactoferrin.
Description
TheHumanLactoferrinproducedfromHumanbreastmilkhasamolecularmassof76.165kDa(calculatedwithoutglycosylation)containing691aminoacidresidues.
Source
Humanbreastmilk.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
LTFproteinfiltered(0.4µm)andlyophilizedin0.5mg/mlin0.05Mphosphatebufferand0.075MNaCl,pH7.4.
Solubility
Itisrecommendedtoadddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Stability
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Aminoacidsequence
GRRRSVQWCAVSQPEATKCFQWQRNMRKVRGPPVSCIKRDSPIQCIQAIAENRADAVTLDGGFIYEAGLAPYKLRPVAAEVYGTERQPRTHYYAVAVVKKGGSFQLNELQGLKSCHTGLRRTAGWNVPIGTLRPFLNWTGPPEPIEAAVARFFSASCVPGADKGQFPNLCRLCAGTGENKCAFSSQEPYFSYSGAFKCLRDGAGDVAFIRESTVFEDLSDEAERDEYELLCPDNTRKPVDKFKDCHLARVPSHAVVARSVNGKEDAIWNLLRQAQEKFGKDKSPKFQLFGSPSGQKDLLFKDSAIGFSRVPPRIDSGLYLGSGYFTAIQNLRKSEEEVAARRARVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTEDCIALVLKGEADAMSLDGGYVYTAGKCGLVPVLAENYKSQQSSDPDPNCVDRPVEGYLAVAVVRRSDTSLTWNSVKGKKSCHTAVDRTAGWNIPMGLLFNQTGSCKFDEYFSQSCAPGSDPRSNLCALCIGDEQGENKCVPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLQNTDGNNNEAWAKDLKLADFALLCLDGKRKPVTEARSCHLAMAPNHAVVSRMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFNDNTECLARLHGKTTYEKYLGPQYVAGITNLKK
CSTSPLLEACEFLRK.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.