Cataloguenumber
PRO-891
Synonyms
Kruppel-likefactor4(gut),EZF,GKLF,EpithelialzincfingerproteinEZF,Gut-enrichedkrueppel-likefactor,endothelialKruppel-likezincfingerprotein.
Introduction
KLF4isatranscriptionfactorthatperformsasbothanactivatorandrepressor.KLF4isexpressedmainlyinerythroidtissuesandfoundmostlyingut.KLF4istakespartinthedifferentiationofepithelialcellsinadditiontoskeletalandkidneydevelopment.
Description
KLF4HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining545aminoacids(11-395)andhavingamolecularmassof58.1kDa.
TheKLF4isfusedtoa159aminoacidHis-CaMTagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheKLF4solution(1mg/ml)contains20mMTris-HClbuffer(pH8.0),1mMDTT,0.1MNaCland10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan80%asdeterminedbySDS-PAGE.
Aminoacidsequence
MAHHHHHHMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAKGSMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLA
AATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTAT
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.