HCVisasmall50nm,enveloped,single-stranded,positivesenseRNAvirusinthefamilyFlaviviridae.
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.Duetolackofproofre
ADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
TheE.coliderivedrecombinantHRPLabeledproteincontainstheHCVNS3immunodominantregions.
HCVNS3,HRPproteinwaspurifiedbyproprietarychromatographictechnique.
HCVNS3,HRPproteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
25mMTris-HClpH8,1mMEDTA,1.5Mureaand50%glycerol.
HCVNS3HRPalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
ImmunoreactivewithseraofHCV-infectedindividuals.
HCVNS3,HRPantigenissuitablefor
ELISAandWesternblots,excellentantigenfordetectionofHCVwithminimalspecificityproblems.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.