HCVisasmall50nm,enveloped,single-stranded,positivesenseRNAvirusinthefamilyFlaviviridae.
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.Duetolackofproofre
ADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
TheE.coliderivedrecombinantBiotinLabeledproteincontainstheHCVNS3immunodominantregions,a.a.1450-1643,22kDa.
TheBiotinlabeledproteinisfusedwithb-galactosidase(114kDa)atN-terminus.
HCVNS3Biotinlabeledproteinwaspurifiedbyproprietarychromatographictechnique.
HCVNS3Biotinlabeledproteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
8Murea,20mMTris-HClpH8.0,10mMb-mercaptoethanol.
HCVNS3Biotinalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
ReactivitywithhumanHCVpositiveserumundetermined.
HCVNS3Biotinlabeledantigenissuitablefor
ELISAandWesternblots,excellentantigenfordetectionofHCVwithminimalspecificityproblems.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.