HCVisasmall50nm,enveloped,single-stranded,positivesenseRNAvirusinthefamilyFlaviviridae.
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.Duetolackofproofre
ADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
TheE.coliderivedrecombinantproteincontainstheHCVNS3immunodominantregions,aminoacids1359-1456.TheproteinisfusedtoaGSTtagatN-Terminus.
HCVNS3Genotype-3proteinwaspurifiedbyproprietarychromatographictechnique.
HCVNS3Genotype-3proteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
1.5Murea,25mMTris-HClpH-8,0.2%Triton-X&50%Glycerol.
HCVNS3Genotype-3althoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
ImmunoreactivewithseraofHCV-infectedindividuals.
HCVNS3Genotype-3antigenissuitablefor
ELISAandWesternblots,excellentantigenfordetectionofHCVwithminimalspecificityproblems.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.