Cataloguenumber
ENZ-016
Synonyms
6PGL,6-Phosphogluconolactonase.
Introduction
PGLSisanenzymeinthesecondsteppentosephosphatepathway.6-PhosphogluconolactonaseiscrucialforthesynthesisofnucleotidesugarsandNADPH,thekeycausefordecreasingpower.PGLStransforms6-phosphogluconolactoneto6-phosphogluconate.
Description
PGLSproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining278aminoacids(1-258a.a.)andhavingamolecularmassof29.7kDa.
PGLSisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
ThePGLSproteinsolution(1mg/1ml)isformulatedin20mMTris-HClbuffer(pH8.0)1mMDTT,100mMNaCland10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Pleaseavoidfreezethawcycles.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIPVFDLLILGVGPDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTVPFEKHSTL
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.