Cataloguenumber
CHM-264
Synonyms
C-X-Cmotifchemokine10,10kDainterferongamma-inducedprotein,Gamma-IP10,IP-10,Interferon-induc
IBLeprotein10,ProteinMob-1,Small-induciblecytokineB10,Cxcl10,Inp10,Mob1,Scyb10.
Introduction
Chemokine(C-X-Cmotif)ligand10(CXCL10)isasmallcytokinebelongingtotheCXCchemokinefamilythatisalsoknownas10kDainterferon-gamma-inducedprotein(?-IP10orIP-10).CXCL10issecretedbyseveralcelltypesinresponsetoIFN-?.Thesecelltypesincludemonocytes,endothelialcellsandfibroblasts.CXCL10hasbeenattributedtoseveralroles,suchaschemoattractionformonocytesandTcells,promotionofTcelladhesiontoendothelialcells,antitumoractivity,andinhibitionofbonemarrowcolonyformationandangiogenesis.ThegeneforCXCL10islocatedonhumanchromosome4inaclusteramongseveralotherCXCchemokines.ThischemokineelicitsitseffectsbybindingtothecellsurfacechemokinereceptorCXCR3.Thethree-dimensionalcrystalstructureofthischemokinehasbeendeterminedunder3differentconditionstoaresolutionofupto1.92A.
Description
IP-10RatRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining77aminoacidsandhavingamolecularmassof8.6kDa.
TheIP-10ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2µmfilteredconcentratedsolutionin1×PBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIP-10insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedIP-10althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionCXCL10shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.
Aminoacidsequence
IPLARTVRCTCIDFHEQPLRPRAIGKLEIIPASLSCPHVEIIATMKKNNEKRCLNPESEAIKSLLKAVSQRRSKRAP.
DeterminedbyitsabilitytochemoattracthCXCR3/HEK293cellsusingaconcentrationrangeof10.0-50.0ng/ml.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.