Cataloguenumber
CYT-441
Synonyms
Transforminggrowthfactor,beta2,cetermin,Glioblastoma-derivedT-cellsuppressorfactor,polyergin,G-TSF,TGF-beta2,TGF-beta-2,transforminggrowthfactorbeta-2,BSC-1cellgrowthinhibitor,TGFB-2.
Introduction
TGFB2isa27.08kDaproteinhavingtwoidentical118aminoacidpeptidechainslinkedbyasingledisulfidebond.TGFB2ispartofafamilyoffiverelatedcytokinesthathaveanextensivevariationofnormalandneoplasticcells,indicatingtheimportanceofthesehomo-dimmerproteinsasmulti-functionalregulatorsofcellularactivity.ThethreemammalianisoformsofTGF-β(TGFb1,TGFb2andTGFb3)signalthroughthesamereceptorandstimulatesimilar
BIOLOGicalresponses.Theyareinvolvedinphysiologicalprocessesasembryogenesis,tissueremodellingandwoundhealing.
Description
TGFB2HumanRecombinantproducedinplantsisahomodimericpolypeptidechaincontaining2x118aminoacidsandhavingatotalmolecularmassof27.08kDa.TheTGFB2isfusedto6xHisTagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Nicotianabenthamiana.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfromaconcentrated(1mg/ml)solutioncontaining50mMTris-HClpH-7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedTGFB2insterile18M-cmH2Onotlessthan1µg/40µl,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedTGFB2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionTGFB2Humanshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
HHHHHHALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIH
EPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASAS
PCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS.
BiologicalActivity
ThebiologicalactivityofTGFB2ismeasuredinculturebyitsabilitytoinhibittheminklungepithelial(Mv1Lu)cellsproliferation.ED50<40ng/ml,correspondingtoaspecificactivityof25,000units/mg.
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.