Prospec/EGF Human, Pichia/CYT-332/1mg,Prospec,,Prospec,prospecbio,ProSpec-Tany,Prospec经销商/代理商 prospecbio产品促销,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Prospec/EGF Human, Pichia/CYT-332/1mg

产品编号：CYT-332

市场价：¥5000.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$250.00

品牌： Prospec

公司：Prospec-Tany

公司分类：

商品介绍

Cataloguenumber

CYT-332

Synonyms

Urogastrone,URG,EGF.

Introduction

Epidermalgrowthfactorhasaprofoundeffectonthedifferentiationofspecificcellsinvivoandisapotentmitogenicfactorforavarietyofculturedcellsofbothectodermalandmesodermalorigin.TheEGFprecursorisbelievedtoexistasamembrane-boundmoleculewhichisproteolyticallycleavedtogeneratethe53-aminoacidpeptidehormonethatstimulatescellstodivide.EGFstimulatesthegrowthofvariousepidermalandepithelialtissuesinvivoandinvitroandofsomefibroblastsincellculture.

Description

EpidermalGrowthFactorHumanRecombinantproducedinPichiaPastorisisasingle,glycosylated,polypeptidechaincontaining51aminoacidsandhavingamolecularmassof6KDa.
TheEGFispurifiedbyproprietarychromatographictechniques.

Source

PichiaPastoris.

PhysicalAppearance

SterileFilteredWhitelyophilized(freeze-dried)powder.

Formulation

Eachlyophilizedmgcontains0.15MNaCl,0.025Msodiumbicarbonate,pH-7.5.

Solubility

ItisrecommendedtoreconstitutethelyophilizedEpidermalGrowthFactorinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.

StABIlity

LyophilizedEpidermalGrowthFactorRecombinantalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow
-18°C.UponreconstitutionEGFshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.

Purity

Greaterthan95.0%asdeterminedby(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.

Aminoacidsequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAsn-Ser-Asp-Ser-Glu,whichagreeswiththesequenceofnativeEGFhuman.
N-terminalmethioninehasbeencompletelyremovedenzymatically.

BIOLOGicalActivity

TheED50,calculatedbythedose-dependantproliferationofmurineBALB/c3T3cells(measuredby3H-thymidineuptake)is<0.1ng/mlcorrespondingtoaspecificactivityof 10,000,000Units/mg.

Proteincontent

Proteinquantitationwascarriedoutbytwoindependentmethods
1.UVspectroscopyat280nmusingtheabsorbencyvalueof2.858astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).

2.AnalysisbyRP-HPLC,usingacalibratedsolutionofEGFasaReferenceStandard.

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。