Wingless-TypeMMTVIntegrationSiteFamily,Member7A,Proto-OncogeneWnt7aProtein,ProteinWnt-7a.
Wingless-TypeMMTVIntegrationSiteFamily,Member7AalsoknownasWNT7AbelongstotheWNTgenefamily,thisfamilyconsistsofstructurallyrelatedgeneswhichencodesecretedsignalingproteins.Theseproteinshavebeenimplicatedinoncogenesisandinseveraldevelopmentalprocesses,includingregulationofcellfateandpatterningduringembryogenesis.WNT7Aisinvolvedinthedevelopmentoftheanterior-posterioraxisinthefemalereproductivetract,andalsoplaysanessentialroleinuterinesmoothmusclepatteringandmaintenanceofadultuterinefunction.Inaddition,MutationsinWNT7AhavebeenassociatedwithFuhrmannandAl-Aw
ADIRaas-RothschildSchinzelphocomeliasyndromes.
WNT7AHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining341aminoacids(32-349a.a)andhavingamolecularmassof38kDa.
WNT7Aisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
EscherichiaColi.
Sterilefilteredcolorlesssolution.
WNT7Aproteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),10%glyceroland0.4MUrea.
Greaterthan85.0%asdeterminedbySDS-PAGE.
MGSSHHHHHHSSGLVPRGSHMGSLGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGLDECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQYHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCTTKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIK
KPLSYRKPMDTDLVYIEKSPNYCEEDPVTGSVGTQGRACNKTAPQASGCDLMCCGRGYNTHQYARVWQCNCKFHWCCYVKCNTCSERTEMYTCK.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.