Cataloguenumber
PRO-1010
Synonyms
Solublecalcium-activatednucleotidase1,SCAN-1,Apyrasehomolog,PutativeMAPK-activatingproteinPM09,PutativeNF-kappa-B-activatingprotein107,CANT1,SHAPY,DBQD,SCAN1.
Introduction
Calcium-activatednucleotidase1(CANT1)isamemberoftheapyrasefamily.TheCANT1proteiniscalcium-dependentnucleotidasewithapreferenceforUDP.Theorderofactivitywithdifferentsubstratesisasfollows:UDP>GDP>UTP>GTP.Moreover,CANT1hasaverylowactivitytowardsADPandanevenloweractivitytowardsATP.Aswellasitdoesn’thydrolyzeAMPandGMP.CANT1’sspecificfunctionisyetunknown,neverthelessitssubstratesareinvolvedinseveralkeysignalingfunctions,includingCa2+release,throughactivationofpyrimidinergicsignaling.MutationsintheCANT1genearelinkedwithDesbuquoisdysplasiawithhandanomalies.
Description
CANT1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining364aminoacids(63-401a.a.)andhavingamolecularmassof40.5kDa.CANT1isfusedtoa25aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
CANT1proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,10%glyceroland50mMNaCl.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMRPAPGRPPTHNAHNWRLGQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.