SinceApril2012,casesoftheMiddleEastRespiratorySyndromeCoronavirus(MERS-CoV)havebeenidentifiedinthefollowingcountries:SaudiAr
ABIa,Qatar,Jordan,theUnitedArabEmirates,Oman,Kuwait,Yemen,Lebanon,Iran,Algeria,theUnitedKingdom,France,Italy,Greece,Germany,theNetherlands,Austria,Tunisia,Egypt,Malaysia,TurkeyandtheUnitedStatesofAmerica.Coronavirusesarethecauseofthecommoncold,SARS(severeacuterespiratorysyndrome)andothersevereillnesseswithhighmortalityrates,allareclassifiedintocoronavirusfamily.MERS-CoVisanewtypeofSARSfoundinthecoronavirusfamilycausingseverepneumoniawithsuddenandseriousrespiratoryillnesswithhighmortalityratesaswell.SinceJanuary27
th2015,theWHOhasreported956humancases,including351deaths.Morecasesofthenewcoronavirusstrainareexpected.Likeinothercoronaviruses,largesurfacespikeglycoproteinisacentralstructuralproteinofthisvirus;itislocatedabovethevirionsurfacetobindandenterintothetargetcell.Spikeproteinhas2domains-S1andS2.TheS1domainisrespons
IBLeforcellulartropismandinteractionwithtargetcell,whiletheS2domainisresponsibleformembranefusion.TheC-terminalofS1domaincontainsareceptorbindingdomain,andisalsoapotentialtargetforvaccinedevelopmentandanantigenfordiagnosis.
RecombinantSARSMERSSpikeS1isapeptidefromaminoacids56-295ofspikeproteinS1producedinE.coliandfusedtoa6xHistagatitsC-terminus(UniProtaccession#AHC74088).SARSMERSispurifiedbyaproprietarychromatographictechnique.
EscherichiaColi.
Sterilefilteredclearsolution.
SARSMERSproteinsolutionissuppliedinPBS,25mMarginineand0.05%sodiumazide.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Proteinis>95%pureasdeterminedby12%PAGE(coomassiestaining).
EAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTIT
KPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYHHHHHH.
Immunoassay.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.