Denguefeverisaffectedby1of4closelylinkedvirus
SEROtypesofthegenusFlavivirus,familyFlaviviridae.Onemighthavedenguefeverinfectedbythedifferentserotypevirusaftertheprimaryinfection.Detectionofparticularantibodiestodenguevirusesisusedforthediagnosisinclinic.Recently,lateralflowrapidtestproductshavebecomeamostsuitableandknownmethodintheclinicaldiagnosis.Though,thereisdifficultyformanufacturerstohavethedengueantigenswithawholecoverageforDengueIgG&IgMrecognitionforall4serotypeinfectionsaswellaswithcolloidgoldbinding
ABIlity.8dengueantigenshavebeenestablishedforthelateralflowproductswhicharewelldefinedfortheircoverageofdengueIgG&IgMrecognition.Theresearchercanselecttheproductsbelowbasedontheirownspecificapplication.
RecombinantDengueVirusSubtype1Envelope45kDa(43-413a.a.)producedinE.coli,containsimportantepitopesfordengueantibodyrecognition.Thisproteinisfusedtoa6xHistag.
E.coli.
Purifiedbyproprietarychromatographictechnique
Proteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
Phosphatebufferedsalinewith25mMarginine.
DengueEnvelope-145kDaalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.Pleasepreventfreezethawcycles.
Canbeusedforlateralflow&ELISAassay.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.