Borreliabelongstoagenusofbacteriaofthespi
Rochetephylum.Borreliacausesborreliosis,whichisazoonotic,vector-bornediseasetransmittedmainlybyticksandsomebylice,dependingonthespecies.Ofthe36knownspeciesofBorrelia,12aredistinguishedtocauseLymediseaseorborreliosisandaretransmittedbyticks.ThemainBorreliaspeciescausingLymediseaseareBorreliaburgdorferi,Borreliaafzelii,andBorreliagarinii.TheBorreliagenusmembershavealinearchromosomewhichisabout900kbpinlengthaswellasanexcessofbothlinearandcircularplasmidsinthe5-220kbpsizerange.Theplasmidsareatypical,ascomparedtomostbacterialplasmids,sincetheycontainmanyparalogoussequences,alargenumberofpseudogenesand,insomecases,essentialgenes.Moreover,anumberoftheplasmidshavefeaturessuggestingthattheyareprophages.
RecombinantBorreliaBurgdorferiBasicMembraneProteinAproducedinE.coliisanon-glycosylated,polypeptidechainhavingacalculatedmolecularmassof36,239Dalton.BorreliaBmpAisexpressedwitha-6xHistagatN-terminusandpurifiedbyproprietarychromatographictechniques.
EscherichiaColi.
SterileFilteredclearsolution.
BorreliaBmpA(1.3mg/1ml)issuppliedin20mMHEPESbufferpH-8.0,6MUreaand200mMNaCl.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Avoidmultiplefreeze-thawcycles.
Greaterthan80.0%asdeterminedbySDS-PAGE.
WesternblotwithLymepositiveplasma.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.