Cataloguenumber
ANT-469
Synonyms
Thymine-DNAGlycosylase,G/TMismatch-SpecificThymineDNAGlycosylase,EC3.2.2.29.
Type
MouseAntiHumanMonoclonal.
Introduction
Thymine-DNAglycosylase(TDG)isamemberoftheTDG/mugDNAglycosylasefamily.TDGisanuclearproteinthatfixesG/TmismatchestoG/Cpairsbyhydrolyzingthecarbon-nitrogenbondbetweenthesugar-phosphatebackboneoftheDNAandthemispairedthymin.Inaddition,TDGremovesuraciland5-bromouracilfrommispairingswithguanine.TheTDGenzymehasanessentialroleincellulardefenseagainstgeneticmutationtriggeredbythespontaneousdeaminationof5-methylcytosineandcytosine.
Clone
PAT2F7A.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Immunogen
Anti-humanTDGmAb,clonePAT2F7A,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwitharecombinanthumanTDGprotein1-410aminoacidspurifiedfromE.coli.
IgSubclass
MouseIgG1heavychainandKappalightchain.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
Forperiodsupto1monthstoreat4°C,forlongerperiodsoftime,storeat-20°C.Preventfreezethawcycles.
StABIlity/ShelfLife
12monthsat-20°C.1monthat4°C.
Applications
Theantibodyhasbeentestedby
ELISA,Westernblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.Recommendedstartingdilutionis1:250.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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