Catalogue number
PRO-522
Synonyms
Glial Filament Protein, GFP.
Introduction
GFP is an intermediate filament. GFP and vimentin are linked to the same filament network; they are localized in the same filaments.mRNAs encoding the glial intermediate filament protein are spatially dispersed in the glial cell cytoplasm close to the location of the glial filaments.
Description
Ultra Pure Glial Filament Protein having a Molecular mass of 52 kDa.
Source
Bovine Spinal Cord.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate buffer pH 7.5, 6M urea, 2mM DTT, 1mM EDTA and 10mM methylammonium chloride.
Solubility
It is recommended to reconstitute the lyophilized GFP in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized GFP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GFP should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Safety Data Sheet
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。