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主营:重组和天然蛋白、细胞因子和生长因子、趋化因子、蛋白激酶、激素和酶、热休克蛋白、病毒抗原、单克隆及多克隆抗体。
℡ 4000-520-616
℡ 4000-520-616
Prospec/Thrombin Bovine/Bovine Thrombin/2000U/PRO-447-2000U-2000U
产品编号:PRO-447-2000U-2000U
市  场 价:¥1300.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$65.00
品      牌: Prospec
公      司:Prospec-Tany
公司分类:
Prospec/Thrombin Bovine/Bovine Thrombin/2000U/PRO-447-2000U-2000U
商品介绍

Cataloguenumber

PRO-447

Synonyms

CoagulationFactorII(Thrombin),Prepro-CoagulationFactorII,EC3.4.21.5,RPRGL2,THPH1,CoagulationFactorII,ProthrombinB-Chain,SerineProtease,Prothrombin,EC3.4.21,PT,F2.

Introduction

Thrombinenzyme(ActivatedFactorIIa)isanimportantclottingpromoterthatcontrolsthetransformationofsolublefibrinogentoinsolubleactivefibrinstrands.Thrombinisacoagulationproteinandaserineprotease(EC3.4.21.5)thatcatalyzesmanycoagulation-relatedreactions.Thrombintriggersfactor-XI,factor-V,Factor-XIIIandfactor-VIII.Thrombinendorsesplateletactivation,usingactivationofprotease-activatedreceptorsontheplatelet.Asaresultofitshighproteolyticspecificity,thrombinhasbecomeanimportantbiochemicalprotein.Thethrombincleavagesite(Leu-Val-Pro-Arg-Gly-Ser)iswidelyusedinlinkerregionsofrecombinantfusionproteinconstructs.Afterthepurificationofthefusionprotein,thrombinisusedtocleavebetweentheArginineandGlycineresiduesofthecleavagesite,efficientlyremovingthepurificationtagfromtheproteinofinterestwithahighdegreeofspecificity.

Source

BovineBlood.

PhysicalAppearance

SterileFilteredlightbrownlyophilized(freeze-dried)powder.

Formulation

LyophilizedfreezedrypowderformulatedwithTrisbuffer,HClandsodiumchloride,PH7.5.

Solubility

ItisrecommendedtoreconstitutethelyophilizedThrombininsterile18MΩ-cmH2O.

BIOLOGicalActivity

ActivitywasdeterminedbythedirectcomparisontoNIHThrombinReferenceStandardwhichuses200ulofdilutedplasma1:1withsalineasasubstrateand100ulofalbuminsolutionbasedonamodificationofthemethodofBiggs.Theclottingdurationsfordeterminingthrombinactivityareintherangeof15–25secondsat37C.Thespecificactivityfoundwas176NIHunitspermg.

StABIlity

LyophilizedThrombinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionThrmbinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。

 

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