Cataloguenumber
PRO-500
Synonyms
Avidin,AVD,AVID.
Introduction
Avidinisatetramericproteinof4identicalsubunits(homotetramer)eachofwhichcanbindtobiotinwithahighdegreeofaffinityandspecificity.Avidinmolecularweightinitstetramericformisestimatedtobebetween66-69kDa.Avidinisproducedintheoviductsofbirds,reptilesandamphibiansandissubsequentlydepositedinthewhitesoftheireggs.Inthechickeneggwhite,avidinmakesuproughly0.05%oftotalprotein(approximately1.8mgperegg).10%ofAvidin’smolecularweightisascribedtocarbohydratecontentwhichiscomposedoffourtofivemannoseandthreeN-acetylglucosamineresidues.Avidinhasatleastthreedistinctiveoligosaccharidestructuraltypewhicharesimilarinstructureandcomposition.Thedissociationconstant(KD)ofavidinisapproximately10-15M,makingitoneofthestrongestknownnon-covalentbonds.
Description
Avidinisaglycosylatedpolypeptidechainhavingamolecularmassof68kDaandcontaining4subunitseachwithabindingsiteforbiotin.TheAvidinispurifiedbyaffinitychromatographictechniques.ThepurificationprocedureensuresminimalcontaminationbyotherproteinsorDNA.Theresultinghighactivityandpurityoftheproductgivesverylownon-specificbinding(NSB).
Source
Hen"seggwhite.
PhysicalAppearance
SterileFilteredwhitelyophilizedpowder.
Solubility
ItisrecommendedtoreconstitutethelyophilizedAvidininsterile18MΩ-cmH2Onotlessthan100µg/mlormorethan10mg/mlsolutions.
LyophilizedAvidinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionAvidinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
14.6units/mgprotein,1unitbinds1µgbiotin.
Applications
AvidinmaybeusedtovisualizebiotinconjugatedmoleculesinELISA,blottingandhistologicaltechniques.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。