Cystatin-C,Cystatin-3,Neuroendocrinebasicpolypeptide,Gamma-trace,Post-gamma-globulin,
CST3,MGC117328.
Cystatinsareasuperfamilyofcysteineproteinaseinhibitorsfoundinbothplantsandanimals.Theycompriseagroupofproteinaseinhibitors,widelydistributedintissuesandbodyfluids,andformtightcomplexeswithcysteineproteasessuchascathepsinB,H,LandS.CystatinC,asecretedmoleculeofthisfamily,isofinterestfrombiochemical,medicineandevolutionarypointsofview.CystatinC,withmolecularweightof13260Da,iscomposedof120aminoacids,lackscarbohydrateandhastwodisulfidebridgeslocatednearthecarboxylterminus.CystatinCisincreasedinpatientswithmalignantdiseases,andisrelatedtotheinsufficiencyofrenalfunctionandappearstobeabetter
Markerthancreatinine.Ontheotherhand,lowlevelsofcystatinCinvolvecausethebreakdownoftheelasticlaminaeand,subsequently,theatherosclerosisanda
BDominalaorticaneurysm.
Cystatin-CProteinisa14.85kDaproteincontaining133aminoacidsfusedtoa8aaN-TerminalHis-tag.
K9Cystatin-Cwasfiltered(0.4µm)andlyophilizedfrom0.5mg/mlsuppliedin20mMTRIS,50mMNaCl,pH7.5.
Adddeionizedwatertoprepareaworkingstocksolutionof0.5mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StorelyophilizedCystatin-CK9at-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.ReconstitutedCystatin-Ccanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
MKHHHHHHASPGAGRRGSRPGAVGGAVDADVGEEGVQQALDFAVREYNRASNDAYHSRALRVLRARKQVVSGMNYFLEVEIGR
TRCTKSQPNLDNCPFHDQPHLMRKTLCSFQIYTVPWLGKTSLVKSSCQDV.
Purityisgreaterthan95%asvisualizedbySDS-PAGE.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.