NRV-216
Humannorovirusisclassifiedintotwogroups,group1&group2.Norwalkvirusisthespecieswhichbelongstogroup1andwasdiscoveredin1968atOhio.Norovirusisafamiliarviruswhichcauseshumangastroenteritiswiththefollowingsymptomsvomiting,diarrheaandsickness.CDCreportrevealedthatthereare19-21millionAmericansinfectedbyNororvirusannuallywith800deaths,1in15peoplewithinfection.Aroundtheworld,thisvirusaffectsabout267millionpeopleandcausesover200,000deathseachyear;theselossesaremostlyinlessdevelopedcountriesandintheveryyoung,elderlyandimmuno-suppressedpopulation,though,mostcasesareself-limitedwithafullrecoverywithinjustafewdays.Norovirusisextremelycontagiousandcanspreadfromhumantohumanthroughinfectedfood,waterorcontaminatedsurfaces.TheoutbreaksusuallyoccurfromNovember-April,whilethepeakisinJanuary.NorovirusisapositivesenseRNAviruswith7.5kbnucleotides,encodingamajorstructuralproteinVP1with50-55kDa.ThefulllengthofVP1capsidcomprisestheinternalN-terminal,Hinge,shell(S)andprotruding(P)domains.Pdomainfrom225to520formsP1-P2-P1structure.Moreover,Pdomainhasareceptorbindingregionwhichrecognizeshumanhisto-bloodgroupantigens(HBGAs).PdomainexpressedinbacteriacanspontaneouslyformaPdimeaswellasaPparticleaggregatedby12Pdimmers.PparticledisplaysanincreasedbindingactivitytoHBGAshigherthanvirus-likeparticle(VLP)formedbythefull-lengthcapsid.Fornorovirusvaccinedevelopment,weconsiderPdomainasagoodcandidate.
TheRecombinantNorovirusGroup-2CapsidP-Domain,E.Coliderived,VA387Straincontainsa.a.from225-520havingaMwof30kDa.Theproteinisfusedtoa6xHistagatN-terminalandpurifiedbychromatographytechniques.
P-domain(225-520a.a.)formsP1-P2-P1structure.P-domainhasareceptorbindingregiontorecognizehumanhisto-bloodgroupantigens(HBGAs).P-domainexpressedin
bacteriacanspontaneouslyformaPdimeandaPparticleaggregatedby12Pdimmers.P-particledisplaysanenhancedbindingactivitytoHBGAshigherthanvirus-likeparticle
(VLP)formedbythefulllengthcapsid.
Phosphatebufferand17mMK2CO3.
TheRecombinantNorovirusGroup-2P-Domainalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
SDS
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。