Cataloguenumber
CYT-778
Synonyms
Neudesin,Cellimmortalization-relatedprotein2,Neuron-derivedneurotrophicfactor,Secretedproteinofunknownfunction,SPUFprotein,NENF,CIR2,SPUF,SCIRP10.
Introduction
NeudesinNeurotrophicFactor(NENF)isamemberofthecytochromeb5family,MAPRsubfamily.NENFcontains1cytochromeb5heme-bindingdomain.NENFexhibitsneurotrophicactivityandactivatesphosphorylationofMAPK1/ERK2,MAPK3/ERK1andAKT1/AKTinprimaryculturedneurons.NENFdoesn’thavemitogenicactivityinprimaryculturedastrocytes.NENFmayplayapartinneuronaldifferentiationandmayhaveatransientinfluenceonneuralcellproliferationinneuralprecursorcells.NENFneurotrophicactivityisincreasedbybindingtoheme.NENFisup-regulatedinimmortalcellsandinducedinestrogenreceptorpositivebreastcancerexpressingprogesteronereceptor.
Description
NENFHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(aa32-172)containing151aminoacidsincludinga10a.aN-terminalHistag.
Source
EscherichiaColi.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
NENFwasfiltered(0.4µm)andlyophilizedfrom0.5mg/mlin0.05Mphosphatebufferand0.075MNaCl,pH7.4.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.NENFisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKHHHHHHASGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF.
SafetyDataSheet
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.