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℡ 4000-520-616
℡ 4000-520-616
Prospec/NENF Human/Neudesin Neurotrophic Factor Human Recombinant/1mg/CYT-778-1mg-1mg
产品编号:CYT-778-1mg-1mg
市  场 价:¥104000.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$5200.00
品      牌: Prospec
公      司:Prospec-Tany
公司分类:
Prospec/NENF Human/Neudesin Neurotrophic Factor Human Recombinant/1mg/CYT-778-1mg-1mg
商品介绍

Cataloguenumber

CYT-778

Synonyms

Neudesin,Cellimmortalization-relatedprotein2,Neuron-derivedneurotrophicfactor,Secretedproteinofunknownfunction,SPUFprotein,NENF,CIR2,SPUF,SCIRP10.

Introduction

NeudesinNeurotrophicFactor(NENF)isamemberofthecytochromeb5family,MAPRsubfamily.NENFcontains1cytochromeb5heme-bindingdomain.NENFexhibitsneurotrophicactivityandactivatesphosphorylationofMAPK1/ERK2,MAPK3/ERK1andAKT1/AKTinprimaryculturedneurons.NENFdoesn’thavemitogenicactivityinprimaryculturedastrocytes.NENFmayplayapartinneuronaldifferentiationandmayhaveatransientinfluenceonneuralcellproliferationinneuralprecursorcells.NENFneurotrophicactivityisincreasedbybindingtoheme.NENFisup-regulatedinimmortalcellsandinducedinestrogenreceptorpositivebreastcancerexpressingprogesteronereceptor.

Description

NENFHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(aa32-172)containing151aminoacidsincludinga10a.aN-terminalHistag.

Source

EscherichiaColi.

PhysicalAppearance

FilteredWhitelyophilized(freeze-dried)powder.

Formulation

NENFwasfiltered(0.4µm)andlyophilizedfrom0.5mg/mlin0.05Mphosphatebufferand0.075MNaCl,pH7.4.

Solubility

Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.NENFisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

StABIlity

Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.

Purity

Greaterthan95.0%asdeterminedbySDS-PAGE.

Aminoacidsequence

MKHHHHHHASGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF.

SafetyDataSheet

SDS

SafetyDataSheet

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。

 

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