Cataloguenumber
CYT-552
Synonyms
Pigmentepithelium-derivedfactor,PEDF,Serpin-F1,SerpinF1,EPC-1,EPC1,PIG35.
Introduction
PEDFisaneurotrophicproteinthatinducesextensiveneuronaldifferentiationinretinoblastomacells.SerpinF1isapotentinhibitorofangiogenesis.EPC1doesn’tundergothestressedtorelaxedconformationtransitioncharacteristicasoftheactiveserpinssinceitexhibitsnoserineproteaseinhibitoryactivity.AqueoushumourlevelofasymmetricdimethylarginineiscorrelatedwithPEDFinhumans.ADMAandPEDFlevelsareincreasedinresponsetoinflammationinuveitis.LackofPEDFexpressionisapotentfactorfortheenhancementoftumorgrowthandangiogenesisinbreastcancer.PEDF&VEGFgenescontributetothedevelopmentofdiabeticretinopathy.PEDFandVEGFstructuralchangesinbloodvesselwallplayanimportantroleinthepathophysiologyofPDpatients.PEDF-overexpressingtumorsexhibitedreducedintratumoralangiogenesis.SerpinF1isanewpromisingapproachforthetreatmentofosteosarcoma.Levelsofthenaturalocularanti-angiogenicfactorSentrinF1(PEDF)isassociatedwithproliferativeretinopathy.VEGFsecretedbyretinalpigmentepithelialcellsupregulatesPEDFexpressionviaVEGFR-1inanautocrinemanner.Sentrin-F1concentrationintheaqueoushumorofdiabeticpatientspredictswhowilldevelopprogressionofretinopathy.PEDFblocksangiogeniceffectsofleptinthroughitsanti-oxidativeproperties.
Description
PEDFHumanRecombinantproducedinE.ColicontaininganaturalvariantM72Tisasingle,non-glycosylated,polypeptidechaincontaining420aminoacids(20-418a.a.)andhavingatotalmolecularmassof46.7kDa.PEDFisfusedtoa20aminoacidHisTagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
ThePEDFsolutioncontains20mMTris-HClbuffer(pH8.0),0.1MNaCl,and20%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMQNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSMSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。