Cataloguenumber
CYT-736
Synonyms
Neuregulin-1,HeregulinAlpha,NRG1-A,NRG1A.
Introduction
Neuregulin/Heregulinisafamilyofstructurallyrelatedpolypeptidegrowthfactorswhicharestemmedfromalternativelysplicedgenes(NRG1,NRG2,NRG3andNRG4).Thusfar,therearemorethan14solubleandtransmembraneproteinsderivedfromtheNRG1gene.ProteolyticprocessingoftheextracellulardomainofthetransmembraneNRG1isoformsreleasesolublegrowthfactors.Theseisoformscontaintheheregulins(HRGs),glialgrowthfactors(GGFs)andsensoryandmotorneuron-derivedfactor(SMDF).AllthesefactorshavetheIgandEGF-likedomain,andareabletobindtoErbB3andErbB4receptortyrosinkinases.Thisbindingstimulateserb3anderb4heterodimerizationwitherb2,promotingintrinsickinaseactivity,whichresultsintyrosinephosphorylation.NRG1isoformsacttoinducethegrowthanddifferentiationofepithelial,neuronal,glial,andothertypesofcells.
Description
RecombinantHumanNeuregulin-1/HeregulinAlpha(EGFDomain)producedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining65aminoacidsandhavingatotalmolecularmassof7.4kDa.NRG1-Aispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2µmfilteredsolutionin20mMPB,pH6.0and150mMNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedNRG1-Ainsterile18M-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedNRG1-Aalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionNRG1-Ashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
SHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCQPGFTGARCTENVPMKVQNQEKAEELYQK.
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofhumanMCF-7cellsislessthan40ng/ml,correspondingtoaspecificactivityof>2.5×104units/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.