Cataloguenumber
CYT-426
Synonyms
HumanPro-NGF,ProNGF,NGFB.
Description
ProNGFisthepro-formoftheneurotrophinnervegrowthfactor.LikethematureproteinproNGFischaracterizedbythecysteineknotmotifconsistingofthreecysteinebridges.Theproteinpredominantlyexistsasanon-covalentlylinkedhomodimer.Pro-NerveGrowthFactorHumanRecombinantproducedinE.Coliisanon-glycosylated,polypeptidechaincontaining224aminoacidsandhavingamolecularmassof50KDa.TheProNGFispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
ProNGFwaslyophilizedfroma0.2µMfilteredsolutionof20mMPBand250mMNaClpH7.2.
Solubility
ItisrecommendedtoreconstitutethelyophilizedProNGFin1xPBStoaconcentrationnolessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedProNGFalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionProNGFshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MEPHSESNVPAGHTIPQAHWTKLQHSLDTALRRARSAPAAAIAARVAGQTRNITVDPRLFKKRRLRSPRVLFSTQPPREAADTQDLDFEVGGAAPFNRTHRSKRSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA.
References
Title:ProNGFinhibitsNGFmediatedTrkAactivationinPC12cells.Publication:JournalofneuRochemistry107.5(2008):1294-1303.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2008.05690.x/epdf
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。