Cataloguenumber
CYT-440
Synonyms
BetaPolypeptide,NGF,NGFB,HSAN5,Beta-NGF,MGC161426,MGC161428.
Introduction
NGF-betahasnervegrowthstimulatingactivityandthecomplexisinvolvedintheregulationofgrowthandthedifferentiationofsympatheticandcertainsensoryneurons.Mutationsinthisgenehavebeenassociatedwithhereditarysensoryandautonomicneuropathy,type5(HSAN5),anddysregulationofthisgene"sexpressionisassociatedwithallergicrhinitis.
Description
NGFbetaMouseproducedinSu
BMAxillaryGlandofGrownMouseisahomodimer,non-glycosylated,polypeptidechaincontaining2identical120aminoacidsandhavingamolecularmassof13,471Daltoneach.TheNGFbetaMouseispurifiedbyadvanced
BIOLOGypurificationtechnology.
Source
SubmaxillaryGlandofGrownMouse.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
TheNGFbetaMousewaslyophilizedfromsolutioncontaining5%mannitoland1%HSA.
Solubility
ItisrecommendedtoreconstitutethelyophilizedMurineNGF-betainsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedMouseBeta-NGFalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionMurineNGF-Betashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
SSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG.
BiologicalActivity
ThemethodusedtotestthebioassayistheNGF-dependentsurvivalofdorsalrootganglianeuronsofchickembryo,correspondingtoaSpecificActivityof500,000IU/mg.
References
VaronS,RaibornC.Dissociation,fractionationandcultureofchickembryosympatheticganglioniccells[J].JNeurocytol,1972;1:211-221.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.