Cataloguenumber
CHM-270
Synonyms
C-Cmotifchemokine17,Small-induc
IBLecytokineA17,Thymusandactivation-regulatedchemokine,CCchemokineTARC,ABCD-2,CCL17,CCL-17,SCYA17,TARC,A-152E5.3,MGC138271,MGC138273.
Introduction
TARC
CDNAencodesa94aminoacidprecursorproteinwitha23aminoacidresiduesignalpeptidethatiscleavedofftogeneratethe71aminoacidresiduematuresecretedprotein.AlongwithCCchemokinefamilymembers,CCL-17hasapproximately24-29%aminoacidsequenceidentitywithRANTES,MIP-1a,MIP-1b,MCP-1,MCP-2,MCP-3andI-309.TARCisexpressedinthymus,andatalowerlevelinthelung,colon,andsmallintestine.TARCisinadditiontransientlyexpressedinstimulatedperipheralbloodmononuclearcells.RecombinantTARChasbeenshowntobechemotacticforTcelllinesbutnotmonocytesorneutrophils.CCL-17wasrecentlyidentifiedtobeaspecificfunctionalligandforCCR4,areceptorthatisselectivelyexpressedonTcells.CCL17isoneofquiteafewCys-Cys(CC)cytokinegenesclusteredontheqarmofchromosome16.CCL17showschemotacticactivityforTlymphocytes,butnotmonocytesorgranulocytes.CCL17bindstochemokinereceptorsCCR4andCCR8.ThischemokineplaysimportantrolesinTcelldevelopmentinthymusaswellasintraffickingandactivationofmatureTcells.
Description
TARCMouseRecombinantproducedinE.Coliisanon-glycosylated,Polypeptidechaincontaining70aminoacidsandhavingamolecularmassof7.9kDa.TheTARCMouseispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfroma0.2µmfilteredconcentratedsolutionin1×PBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedTARCinsterile18M-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedTARCalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18C.UponreconstitutionTARCshouldbestoredat4Cbetween2-7daysandforfutureusebelow-18C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ARATNVGRECCLDYFKGAIPIRKLVSWYKTSVECSRDAIVFLTVQGKLICADPKDKHVKKAIRLVKNPRP.
TheED50asdeterminedbyitsabilitytochemoattractBaF3mousepro-BcellstransfectedwithhumanCCR4istypically2-10ng/ml.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.