Cataloguenumber
CHM-342
Synonyms
Smallinduc
IBLecytokineA5,CCL5,T-cell-specificRANTESprotein,SIS-delta,Tcell-specificproteinP228,TCP228,chemokine(C-Cmotif)ligand5,SISd,SCYA5,RANTES,D17S136E,MGC17164.
Introduction
RegulateduponActivation,NormalT-cellExpressed,andSecretedorRANTESisan8kDaproteinclassifiedasachemotacticcytokineorchemokine.IthasrecentlybeenrenamedCCL5.RANTESischemotacticforTcells,eosinophilsandbasophilsandplaysanactiveroleinrecruitingleukocytesintoinflammatorysites.Withthehelpofparticularcytokines(i.e.IL-2andIFN-?)thatarereleasedbyTcells,RANTESalsoinducestheproliferationandactivationofcertainnaturalkiller(NK)cellstoformCHAK(CC-Chemokine-activatedkiller)cells.ItisalsoaHIV-suppressivefactorreleasedfromCD8+Tcells.Thischemokinehasbeenlocalizedtochromosome17inhumans.
Description
RantesMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining68aminoacidsandhavingamolecularmassof7.8kDa.TheMouseRantesispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Filtered(0.2µm)andlyophilizedfromasolutionin2×PBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedRantesinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedMouseRantesalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionMouseCCL5shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
SPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNRQVCANPEKKWVQEYINYLEMS.
Theactivityisdeterminedbytheabilitytochemoattracttotalhumanlymphocytespopulationataconcentrationbetween1-10ng/ml.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.