Cataloguenumber
CHM-333
Synonyms
SmallinducIBLecytokineB9,CXCL9,GammaINF-inducedmonokine,MIG,chemokine(C-X-Cmotif)ligand9,CMK,Humig,SCYB9,crg-10,monokineinducedbygamma-INF.
Introduction
Chemokine(C-X-Cmotif)ligand9(CXCL9)isasmallcytokinebelongingtotheCXCchemokinefamilythatisalsoknownasMonokineinducedbygammaINF(MIG).CXCL9isaT-cellchemoattractant,whichisinducedbyIFN-?.ItiscloselyrelatedtotwootherCXCchemokinescalledCXCL10andCXCL11,whosegenesarelocatednearthegeneforCXCL9onhumanchromosome4.CXCL9,CXCL10andCXCL11allelicittheirchemotacticfunctionsbyinteractingwiththechemokinereceptorCXCR3.
Description
MIG(monokineinducedbygamma-INF)HumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining103aminoacidsandhavingamolecularmassof11700Dalton.TheMIGispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentrated(1.0mg/ml)solutionin20mMPB,pH7.4,50mMNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedMIGinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedMIGalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionCXCL9shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
TPVVRKGRCSCISTNQGTIHLQSLKDLKQFAPSPSCEKIEIIATLKNGVQTCLNPDSADVKELIKKWEKQVSQKKKQKNGKKHQKKKVLKVRKSQRSRQKKTT.
DeterminedbyitsabilitytochemoattracthumanperipheralbloodT-Lymphocytesusingaconcentrationrangeof10-100ng/mlcorrespondingtoaSpecificActivityof10,000-100,000IU/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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