Cataloguenumber
CYT-505
Synonyms
OBProtein,ObesityProtein,OBS,Obesityfactor.
Introduction
A16-kDapeptidehormonesecretedfromwhite
ADIpocytesandimplicatedintheregulationoffoodintakeandenergybalance.Leptinprovidesthekeyafferentsignalfromfatcellsinthefeedbacksystemthatcontrolsbodyfatstores.
Description
LeptinChickenRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining145aminoacidsandhavingamolecularmassof16kDa.TheLeptinispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.0045mMNaHCO3.
Solubility
ItisrecommendedtoreconstitutethelyophilizedLeptininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedLeptinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionLeptinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAla-Val-Pro-Cys-Gln.
BiologicalactiveasevidencedbyinducingproliferationofBAF/3cellsstablytransfectedwiththelongformofhumanleptinreceptor.Itsactivityishowever5-10foldlowerascomparedtomammalianleptins.
Proteincontent
Proteinquantitationwascarriedoutbytwoindependentmethods1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.19astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingcalibratedsolutionofLeptinChickenasaReferenceStandard.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.