Prospec/pGH 22kDa Human/Growth Hormone Placental 22kDa Human Recombinant/50µg/CYT-235-50µg-50µg,Prospec,,Prospec,prospecbio,ProSpec-Tany,中国Prospec官网，prospecbio价格更新 ProSpec病毒抗原,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Prospec/pGH 22kDa Human/Growth Hormone Placental 22kDa Human Recombinant/50µg/CYT-235-50µg-50µg

产品编号：CYT-235-50µg-50µg

市场价：¥3600.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>生长因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$180.00

品牌： Prospec

公司：Prospec-Tany

公司分类：

商品介绍

Cataloguenumber

CYT-235

Synonyms

GHL,GHV,GH-V,hGH-V,PGH.

Introduction

GHisamemberofthesomatotropin/prolactinfamilyofhormoneswhichplayanimportantroleingrowthcontrol.Thegene,alongwithfourotherrelatedgenes,islocatedatthegrowthhormonelocusonchromosome17wheretheyareinterspersedinthesametranscriptionalorientation;anarrangementwhichisthoughttohaveevolvedbyaseriesofgeneduplications.Thefivegenessharearemarkablyhighdegreeofsequenceidentity.Alternativesplicinggeneratesadditionalisoformsofeachofthefivegrowthhormones,leADIngtofurtherdiversityandpotentialforspecialization.Thisparticularfamilymemberisexpressedinthepituitarybutnotinplacentaltissueasisthecasefortheotherfourgenesinthegrowthhormonelocus.Mutationsinordeletionsofthegeneleadtogrowthhormonedeficiencyandshortstature.

Description

PlacentalHGH22kDaRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining192aminoacidsandhavingamolecularmassof22367Dalton.PredictedpI=7.80.PlacentalGrowthHormonehasdimishedlactogenic(prolactinreceptormediated)activitycharacteristictopituitaryGHs.GHPlacentalHumanRecombinantispurifiedbyproprietarychromatographictechniques.

Source

EscherichiaColi.

PhysicalAppearance

SterileFilteredWhitelyophilized(freeze-dried)powder.

Formulation

Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.0045mMNaHCO3previouslyadjustedpH8-9.

Solubility

ItisrecommendedtoreconstitutethelyophilizedPlacentalHGHin0.4%NaHCO3orwateradjustedtopH9,notlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.

StABIlity

LyophilizedGHplacentalalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionandfiltersterilizationHGHplacentalcanbestoredat4°Cforupto4weeks.Forlongtermstorageandmoredilutedsolutionsitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

Purity

Greaterthan98.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.

Aminoacidsequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAla-Phe-Pro-Thr-Ile.

Proteincontent

Proteinquantitationwascarriedoutbytwoindependentmethods1.UVspectroscopyat280nmusingtheabsorbencyvalueof1.18astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingacalibratedsolutionofGH-22K-placentalasaReferenceStandard.

References

Title:ProteinQuantificationbyIsotopeDilutionMassSpectrometryofProteolyticFragments:CleavageRateandAccuracy.Publication:Anal.Chem.,2008,80(11),pp4154–4160DOI:10.1021/ac7024738

PublicationDate(Web):April30,2008

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。